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NEET Biology · Biotechnology & its Applications · Set 1 (Q1-25)

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  1. 1.
    Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:
    1. (1)Mutated gene completely and clearly appears on a photographic film.
    2. (2)Mutated gene does not appear on a photographic film as the probe has no complimentarity with it.
    3. (3)Mutated gene does not appear on photographic film as the probe has complementarity with it.
    4. (4)Mutated gene partially appears on a photographic film.
  2. 2.
    Genetic engineering has been successfully used for producing
    1. (1)Transgenic mice for testing safety of polio vaccine before used in humans.
    2. (2)Transgenic models for studying new treatments for certain cardiac diseases.
    3. (3)Transgenic cow Rosie which produces high fat milk for making ghee.
    4. (4)Animals like bulls for farm work as they have super power.
  3. 3.
    Which of the following Bt crops is being grown in India by the farmers?
    1. (1)Maize
    2. (2)Cotton
    3. (3)Brinjal
    4. (4)Soybean
  4. 4.
    Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein
    1. (1)Binds with epithelial cells of midgut of the insect pest ultimately killing it.
    2. (2)Is coded by several genes including the gene cry.
    3. (3)Is activated by acid pH of the foregut of the insect pest.
    4. (4)Does not kill the carrier bacterium which is itself resistant to this toxin.
  5. 5.
    Which one of the following is used as vector for cloning genes into higher organisms?
    1. (1)Baculovirus
    2. (2)Salmonella typhimurium
    3. (3)Rhizopus nigricans
    4. (4)Retrovirus
  6. 6.
    In Bt cotton the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to
    1. (1)Alkaline pH of the insect gut
    2. (2)Acidic pH of the insect gut
    3. (3)Action of gut microorganisms
    4. (4)Presence of conversion factors in insect gut
  7. 7.
    Which part of the tobacco plant is infected by Meloidogyne incognita?
    1. (1)Flower
    2. (2)Leaf
    3. (3)Stem
    4. (4)Root
  8. 8.
    Some of the characteristics of Bt cotton are
    1. (1)Long fiber and resistance to aphids.
    2. (2)Medium yield, long fiber and resistance to beetle pests.
    3. (3)High yield and production of toxic protein crystals which kill dipteran pests.
    4. (4)High yield and resistance to bollworms.
  9. 9.
    The crops engineered for glyphosate are resistant/tolerant to
    1. (1)Fungi
    2. (2)Bacteria
    3. (3)Insects
    4. (4)Herbicides
  10. 10.
    The process of RNA interference has been used in the development of plants resistant to
    1. (1)Fungi
    2. (2)Viruses
    3. (3)Insects
    4. (4)Nematodes
  11. 11.
    Read the following four statements (A to D) about certain mistakes in two of them.
    (A) The first transgenic buffalo, Rosie produced milk which was human alpha-lactalbumin enriched.
    (B) Restriction enzymes are used in isolation of DNA from other macromolecules.
    (C) Downstream processing is one of the steps of rDNA technology.
    (D) Disarmed pathogen vectors are also used in transfer of rDNA into the host.
    Which of the two statements have mistakes?
    1. (1)(B) and (C)
    2. (2)(C) and (D)
    3. (3)(A) and (C)
    4. (4)(A) and (B)
  12. 12.
    Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis is resistant to
    1. (1)Fungal diseases.
    2. (2)Plant nematodes
    3. (3)Insect predators
    4. (4)Insect pests
  13. 13.
    Which body of the Government of India regulates GM research and safety of introducing GM organism for public services?
    1. (1)Bio-safety committee
    2. (2)Indian Council of Agricultural Research
    3. (3)Genetic Engineering Approval Committee
    4. (4)Research Committee on Genetic manipulation
  14. 14.
    Which of the following statements is not correct?
    1. (1)The proinsulin has an extra peptide called C-peptide
    2. (2)The functional insulin has and chains linked together by hydrogen bonds.
    3. (3)Genetically engineered insulin is produced in E.coli.
    4. (4)In man insulin is synthesised as a proinsulin.
  15. 15.
    Silencing of mRNA has been used in producing transgenic plants resistant to
    1. (1)Bollworms
    2. (2)Nematodes
    3. (3)White rusts
    4. (4)Bacterial blights
  16. 16.
    The two polypeptides of human insulin are linked together by:
    1. (1)Hydrogen bonds
    2. (2)Phosphodiester bond
    3. (3)Covalent bond
    4. (4)Disulphide bridges
  17. 17.
    Which of the following is not an application of PCR (Polymerase Chain Reaction)?
    1. (1)Gene amplification
    2. (2)Purification of isolated protein
    3. (3)Detection of gene mutation
    4. (4)Molecular diagnosis
  18. 18.
    Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?
    1. (1)Enzyme Linked Immuno-Sorbent Assay (ELISA) technique
    2. (2)Recombinant DNA Technology
    3. (3)Serum and Urine analysis
    4. (4)Polymerase Chain Reaction (PCR) technique
  19. 19.
    The introduction of t-DNA into plants involves
    1. (1)Altering the pH of soil, then heat-shocking the plants.
    2. (2)Exposing the plants to cold for a brief period.
    3. (3)Allowing the plant roots to stand in water.
    4. (4)Infection of the plant by Agrobacterium tumefaciens.
  20. 20.
    The first human hormone produced by recombinant DNA technology is
    1. (1)Insulin
    2. (2)estrogen
    3. (3)Thyroxin
    4. (4)Progesterone
  21. 21.
    Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)
    1. (1)A particular hormone
    2. (2)An antioxidant
    3. (3)A toxic protein
    4. (4)Both sense and anti-sense RNA
  22. 22.
    DNA or RNA segment tagged with a radioactive molecule is called
    1. (1)Vector
    2. (2)Probe
    3. (3)Clone
    4. (4)Plasmid
  23. 23.
    When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:
    1. (1)Gene therapy
    2. (2)Molecular diagnosis
    3. (3)Safety testing
    4. (4)Biopiracy
  24. 24.
    The adenosine deaminase deficiency results into:
    1. (1)Parkinson's disease
    2. (2)Digestive disorder
    3. (3)Addison's disease
    4. (4)Dysfunction of Immune system
  25. 25.
    The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
    1. (1)Non-recombinant bacteria containing β-galactosidase.
    2. (2)Insertional inactivation of β-galactosidase in non-recombinant bacteria.
    3. (3)Insertional inactivation of β-galactosidase in recombinant bacteria.
    4. (4)Inactivation of glycosidase enzyme in recombinant bacteria.