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NEET Biology · Biotechnology: Principles & Processes · Set 1 (Q1-30)

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  1. 1.
    What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in boll worm?
    1. (1)Body temperature
    2. (2)Moist surface of midgut
    3. (3)Alkaline pH of gut
    4. (4)Acidic pH of stomach
  2. 2.
    Which of the following is a restriction endonuclease?
    1. (1)Hind II
    2. (2)Protease
    3. (3)DNase I
    4. (4)RNase
  3. 3.
    DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as:
    1. (1)Bright orange bands
    2. (2)Dark red bands
    3. (3)Bright blue bands
    4. (4)Yellow bands
  4. 4.
    Stirred-tank bioreactors have been designed for
    1. (1)Addition of preservatives of the product
    2. (2)Purification of the product
    3. (3)Ensuring anaerobic conditions in the culture vessel
    4. (4)Availability of oxygen throughout the process
  5. 5.
    In gel electrophoresis, separated DNA fragments can be visualized with the help of:
    1. (1)Ethidium bromide in UV radiation
    2. (2)Acetorarmine in UV radiation
    3. (3)Ethidium bromide in infrared radiation
    4. (4)Acetocarmine in bright blue light
  6. 6.
    Which of the following is not a component of downstream processing?
    1. (1)Purification
    2. (2)Preservation
    3. (3)Expression
    4. (4)Separation
  7. 7.
    Which of the following is not a feature of the plasmids?
    1. (1)Independent replication
    2. (2)Circular structure
    3. (3)Transferable
    4. (4)Single - stranded
  8. 8.
    Use of bioresources by multinational companies and organisations without authorisation from the concerned country and its people is called
    1. (1)Bio-infringement
    2. (2)Biopiracy
    3. (3)Biodegradation
    4. (4)Bioexploitatio
  9. 9.
    Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?
    1. (1)Indian Council of Agricultural Research
    2. (2)Genetic Engineering Approval Committee
    3. (3)Research Committee on Genetic Manipulation
    4. (4)Bio-safety committee
  10. 10.
    Which one of the following represents a palindromic sequence in DNA?
    1. (1)5' -CATTAG- 3'
      3' -GATAAC- 5'
    2. (2)5' -GATACC- 3'
      3' -CCTAAG- 5'
    3. (3)5' -GAATTC- 3'
      3' -CTTAAG- 5'
    4. (4)5' -CCAATG- 3'
      3' -GAATCC- 5'
  11. 11.
    In genetic engineering, the antibiotics are used :-
    1. (1)as sequences from where replication starts
    2. (2)to keep the cultures free of infection
    3. (3)as selectable markers
    4. (4)to select healthy vectors
  12. 12.
    Given below is a sample portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it?
    5’_GAATTC_3’
    3’_CTTAAG_5’
    1. (1)Deletion mutation
    2. (2)Start codon at the 5’ end
    3. (3)Palindromic sequence of base pairs
    4. (4)Replication completed
  13. 13.
    The introduction of T-DNA into plants involves :
    1. (1)Allowing the plant roots to stand in water
    2. (2)Infection of the plant by Agrobacterium tumefaciens
    3. (3)Altering the pH of the soil, then heat shocking the plants
    4. (4)Exposing the plants to cold for a brief period
  14. 14.
    Following statements describe the characteristics of the enzyme Restriction endonuclease. Identify the incorrect statement.
    1. (1)The enzyme cuts DNA molecule at identified position within the DNA
    2. (2)The enzyme binds DNA at specific sites and cuts only one of the two strands.
    3. (3)The enzyme cuts the sugar-phosphate backbone at specific sites on each strand.
    4. (4)The enzyme recognizes a specific palindromic nucleotide sequence in the DNA
  15. 15.
    When gene targetting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as :
    1. (1)Biopiracy
    2. (2)Gene therapy
    3. (3)Molecular diagnosis
    4. (4)Safety testing
  16. 16.
    In recombinant DNA technology antibiotics are used:
    1. (1)To detect alien DNA
    2. (2)To impart disease-resistance to the host plant
    3. (3)As selectable markers
    4. (4)To keep medium bacteria-free
  17. 17.
    DNA fragments generated by the restriction endonucleases in a chemical reaction can be separated by:
    1. (1)Restriction mapping
    2. (2)Centrifugation
    3. (3)Polymerase chain reaction
    4. (4)Electrophoresis
  18. 18.
    Stirred-tank bioreactors have been designed for:
    1. (1)availability of oxygen throughout the process
    2. (2)ensuring anaerobic conditions in the culture vessel
    3. (3)purification of product
    4. (4)addition of preservatives to the product
  19. 19.
    The first recombinant DNA was constructed by linking an antibiotic resistant gene with native plasmid of
    1. (1)Escherichia coli
    2. (2)Salmonella typhimurium
    3. (3)Bacillus thuringiensis
    4. (4)Rhizobium japonicum
  20. 20.
    A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:
    1. (1)Okazaki sequences
    2. (2)Palindromic Nucleotide sequences
    3. (3)Poly (A) tail sequences
    4. (4)Degenerate primer sequence
  21. 21.
    The laws and rules to prevent unauthorised exploitation of bio-resources are termed as-
    1. (1)Biopatenting
    2. (2)Bioethics
    3. (3)Bioengineering
    4. (4)Biopiracy
  22. 22.
    Which kind of therapy was given in 1990 to a four year old girl with adenosine deaminase (ADA) deficiency?
    1. (1)Immunotherapy
    2. (2)Radiation therapy
    3. (3)Gene therapy
    4. (4)Chemotherapy
  23. 23.
    The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of:
    1. (1)Inactivation of glycosidase enzyme in recombinant bacteria
    2. (2)Non-recombinant bacteria containing beta-galactosidase
    3. (3)Insertional inactivation of alpha-galactosidase in non-recombinant bacteria
    4. (4)Insertional inactivation of alpha-galactosidase in recombinant bacteria
  24. 24.
    In gene gun method used to introduce alien DNA into host cells, microparticles of metal are used.
    1. (1)Silver
    2. (2)Copper
    3. (3)Zinc
    4. (4)Tungsten or gold
  25. 25.
    The taq polymerase enzyme is obtained from :
    1. (1)Thermus aquaticus
    2. (2)Thiobacillus ferroxidans
    3. (3)Bacillus subtilis
    4. (4)Pseudomonas putida
  26. 26.
    First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:
    1. (1)Adensosine deaminase
    2. (2)Thermostable DNA polymerase
    3. (3)Hind II
    4. (4)EcoRI
  27. 27.
    The genetic defect - adenosine deaminase (ADA) deficiency may be cured permanently by :-
    1. (1)Enzyme replacement therapy
    2. (2)Periodic infusion of genetically engineered lymphocytes having functional ADA cDNA
    3. (3)Administering adenosine deaminase activators
    4. (4)Introducing bone marrow cells producing ADA into cells at early embryonic stages
  28. 28.
    Select the correct statement from the following :
    1. (1)Gel electrophoresis is used for amplification of a DNA segment.
    2. (2)The polymerase enzyme joins the gene of interest and the vector DNA.
    3. (3)Restriction enzyme digestions are performed by incubating purified DNA molecules with the restriction enzymes of optimum conditions.
    4. (4)PCR is used for isolation and separation of gene of interest.
  29. 29.
    Which vector can clone only a small fragment of DNA?
    1. (1)Bacteria artificial chromosome
    2. (2)Yeast artificial chromosome
    3. (3)Plasmid
    4. (4)Cosmid
  30. 30.
    Maximum number of existing transgenic animals is of :-
    1. (1)Fish
    2. (2)Mice
    3. (3)Cow
    4. (4)Pig