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Set 2 of 4 · Questions 31-60 of 120

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NEET Biology · Biotechnology: Principles & Processes · Set 2 (Q31-60)

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  1. 31.
    The process of separation and purification of expressed protein before marketing is called:
    1. (1)Downstream processing
    2. (2)Bioprocessing
    3. (3)Postproduction processing
    4. (4)Upstream processing
  2. 32.
    Agarose extracted from sea weeds finds use in:-
    1. (1)Spectrophotometry
    2. (2)Tissue Culture
    3. (3)PCR
    4. (4)Gel electrophoresis
  3. 33.
    During the process of gene amplification using PCR, if very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
    1. (1)Annealing
    2. (2)Extension
    3. (3)Denaturation
    4. (4)Ligation
  4. 34.
    Restriction endonucleases are enzymes which :
    1. (1)restrict the action of the enzyme DNA polymerase
    2. (2)remove nucleotides from the ends of the DNA molecule
    3. (3)make cuts at specific positions within the DNA molecule
    4. (4)recognize a specific nucleotide sequence for binding of DNA ligase
  5. 35.
    Upon exposure to UV radiation, DNA stained with ethidium bromide will show
    1. (1)Bright orange colour
    2. (2)Bright red colour
    3. (3)Bright blue colour
    4. (4)Bright yellow colour
  6. 36.
    An enzyme catalysing the removal of nucleotides from ends of DNA is:
    1. (1)DNA ligase
    2. (2)Endonuclease
    3. (3)Exonuclease
    4. (4)Protease
  7. 37.
    In genetic engineering, a DNA segment (gene) of interest, is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select the correct option about which one or more of these can be used as a vector/ vectors.
    (A) A bacterium
    (B) Plasmid
    (C) Plasmodium
    (D) Bacteriophage
    1. (1)(A), (B) and (D) only
    2. (2)(A) only
    3. (3)(A) and (C) only
    4. (4)(B) and (D) only
  8. 38.
    Which of the following restriction enzymes produces blunt ends?
    1. (1)Xho I
    2. (2)Hind III
    3. (3)Sal I
    4. (4)Eco RV
  9. 39.
    There is a restriction endonuclease called EcoRI. What does "co" part in it stand for?
    1. (1)Colon
    2. (2)Coelom
    3. (3)Coenzyme
    4. (4)Coli
  10. 40.
    The cutting of DNA at specific locations became possible with the discovery of :
    1. (1)Ligases
    2. (2)Restriction enzymes
    3. (3)Probes
    4. (4)Selectable markers
  11. 41.
    The cutting of DNA at specific locations is possible with the discovery of:
    1. (1)Probes
    2. (2)Selectable markers
    3. (3)Ligases
    4. (4)Restriction enzymes
  12. 42.
    Which one of the following techniques made it possible to genetically engineer living organisms?
    1. (1)Recombinant DNA techniques
    2. (2)X-ray diffraction
    3. (3)Heavier isotope labeling
    4. (4)Hybridization
  13. 43.
    DNA precipitation out of a mixture of biomolecules can be achieved by treatment with:
    1. (1)Isopropanol
    2. (2)Chilled ethanol
    3. (3)Methanol at room temperature
    4. (4)Chilled chloroform
  14. 44.
    The correct order of steps in Polymerase Chain Reaction (PCR) is
    1. (1)Extension, Denaturation, Annealing
    2. (2)Annealing, Extension, Denaturation
    3. (3)Denaturation, Extension, Annealing
    4. (4)Denaturation, Annealing, Extension
  15. 45.
    Separation of DNA, fragments is done by a technique known as :
    1. (1)Polymerase Chain Reaction
    2. (2)Recombinant technology
    3. (3)Southern blotting
    4. (4)Gel electrophoresis
  16. 46.
    The DNA fragments separated on an agarose gel can be visualised after staining with:
    1. (1)Acetocarmine
    2. (2)Aniline blue
    3. (3)Ethidium bromide
    4. (4)Bromophenol blue
  17. 47.
    The sequence that controls the copy number of the linked DNA in the vector, is termed:
    1. (1)Ori site
    2. (2)Palindromic sequence
    3. (3)Recognition site
    4. (4)Selectable marker
  18. 48.
    A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using:
    1. (1)Polymerase-III
    2. (2)Ligase
    3. (3)Eco RI
    4. (4)Taq polymerase
  19. 49.
    Which of the following are used in gene cloning?
    1. (1)Nucleosides
    2. (2)Lomasomes
    3. (3)Mesosomes
    4. (4)Plasmids
  20. 50.
    Which one of the following equipments is essentially required for growing microbes on a large scale, for industrial production of enzymes?
    1. (1)BOD incubator
    2. (2)Sludge digester
    3. (3)Industrial oven
    4. (4)Bioreactor
  21. 51.
    Cry1 endotoxins obtained from Bacillus thuringiensis are effective against :-
    1. (1)Flies
    2. (2)Nematodes
    3. (3)Boll worms
    4. (4)Mosquitoes
  22. 52.
    A single stranded DNA or RNA, tagged with a radioactive molecule is allowed to hybridise to it complementary DNA in a clone of cells followed by detection using
    1. (1)Polymerase chain reaction
    2. (2)Biolistic or gene gun
    3. (3)Autroradiography
    4. (4)DNA fingerprinting
  23. 53.
    Transposons can be used during which one of the following?
    1. (1)Gene silencing
    2. (2)Autoradiography
    3. (3)Gene sequencing
    4. (4)Polymerase Chain Reaction
  24. 54.
    Which of the following is not a desirable feature of a cloning vector?
    1. (1)Presence of two or more recognition sites
    2. (2)Presence of origin of replication
    3. (3)Presence of a marker gene
    4. (4)Presence of single restriction enzyme site
  25. 55.
    Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
    1. (1)Retrovirus
    2. (2)Ti plasmid
    3. (3)Lambda phage
    4. (4)pBR 32
  26. 56.
    For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very useful for early detection?
    1. (1)Western Blotting Technique
    2. (2)Southern Blotting Technique
    3. (3)ELISA Technique
    4. (4)Hybridization Technique
  27. 57.
    A transgenic food crop which may help in solving the problem of night blindness in developing countries is :-
    1. (1)Starlink maize
    2. (2)Bt Soybean
    3. (3)Golden rice
    4. (4)Flavr Savr tomatoes
  28. 58.
    The DNA molecules to which the gene of interest is integrated for cloning is called :
    1. (1)Carrier
    2. (2)Transformer
    3. (3)Vector
    4. (4)Template
  29. 59.
    RNA interference is used for which of the following purposes in the field of biotechnology?
    1. (1)to develop a plant tolerant to abiotic stresses
    2. (2)to develop a pest resistant plant against infestation by nematode
    3. (3)to enhance the mineral usage by the plant
    4. (4)to reduce post harvest losses
  30. 60.
    The crops engineered for glyphosate are resistant/tolerant to :-
    1. (1)Bacteria
    2. (2)Insects
    3. (3)Herbicides
    4. (4)Fung