Biotechnology: Principles & Processes PYQs Set 4 (PDF)

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Mere Teacher · Previous Year Questions NEET Biology · Biotechnology: Principles & Processes · Set 4 (Q91-120)
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91. $Which one of the following is commonly used in transfer of foreign DNA into crop plants?$ (1) $Penicillium expansum$ (2) $Trichoderma harzianum$ (3) $Meloidogyme incognita$ (4) $Agrobacterium tumefaciens$ 92. $Milk of transgenic 'Cow Rosie' was nutritionally more balanced product for human babies than natural cow milk because it contained:$ (1) $Human protein alpha-1-antitrypsin$ (2) $Human alpha-lactalbumin$ (3) $Human insulin-like growth factor$ (4) $Human enzyme Adenosine Deaminase (ADA)$ 93. $A gene whose expression helps to identify transformed cell is known as:$ (1) $Vector$ (2) $Plasmid$ (3) $Structural gene$ (4) $Selectable marker$ 94. $An improved variety of transgenic basmatirice :$ (1) $is completely resistant to all insect pests and diseases of paddy$ (2) $gives high yield but has no charactristic aroma$ (3) $does not require chemical fertilizers and growth hormones$ (4) $gives a high yield and is rich in vitamin A$ 95. $Plasmid pBR322 has Pstl restriction enzyme site within gen am p^{R} that confers ampicillin resistance, If this enzyme is used for inserting a gene for β - galactoside production and the recombinant plasmid is inserted in an E.coli strain$ (1) $The transformed cells will have the ability to resist ampicillin as well as produce β -galactoside.$ (2) $It will lead to lysis of host cell.$ (3) $It will be able to produce a novel protein with dual ability.$ (4) $It will not be able to confer ampicillin resistance to host cell.$ 96. $Genetically engineered insulin for human is produced from:$ (1) $Pseudomonas putida$ (2) $Bacillus thuringiensis$ (3) $Rhizobium meliloti$ (4) $Escherichia coli$ 97. $The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of which of the following organism?$ (1) $Bacillus thuringiensis$ (2) $Salmonella typhimurium$ (3) $Agrobacterium tumefaciens$ (4) $Escherichia coli$ 98. $DNA or RNA segment tagged with a radiative molecule is called:$ (1) $Clone$ (2) $Plasmid$ (3) $Vector$ (4) $Probe$ 99. $Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin 'A' deficiency?$ (1) $Golden rice$ (2) $Bt-Brinjal$ (3) $Flaver Savr 'tomato$ (4) $Canolla$ 100. $Which of the following is true for Golden rice?$ (1) $It is Vitamin A enriched, with a gene from daffodil$ (2) $It is pest resistant, with a gene from Bacillus thuringiensis$ (3) $It is drought tolerant, developed using Agrobacterium vector$ (4) $It has yellow grains, because of a gene introduced from a primitive variety of rice$ 101. $Which vector can clone only a small fragment of DNA?$ (1) $Bacterial artificial chromosome$ (2) $Yeast artificial chromosome$ (3) $Plasmid$ (4) $Cosmid$ 102. $Which of the following is not a cloning vector?$ (1) $Probe$ (2) $BAC$ (3) $YAC$ (4) $pBR322$ 103. $The DNA molecule to which the gene of interest is integrated for cloning is called:$ (1) $Vector$ (2) $Template$ (3) $Carrier$ (4) $Transformer$ 104. $Human insulin is being commercially produced from a transgenic species of :-$ (1) $Mycobacterium$ (2) $Rhizobium$ (3) $Saccharomyces$ (4) $Escherichi$ 105. $Golden rice is a genetically modified crop plant where the incorporated gene is meant for biosynthesis of :$ (1) $Vitamin A$ (2) $Vitamin B$ (3) $Vitamin C$ (4) $Omega 3$ 106. $A 'new variety of rice was patented by a foreign company though such varieties have been present in India for a long time. This is related to$ (1) $Co-667$ (2) $Sharbati Sonora$ (3) $Lerma Rojo$ (4) $Basmati$ 107. $Given below is a sample of a portion of DNA strand giving the base sequence on the opposite strands. What is so special shown in it? 5'______GAATTC_______3' 3'______CTTAAG_______5'$ (1) $Replication completed$ (2) $Dejection mutation$ (3) $Start codon at the 5' end$ (4) $Palindromic sequence of base pairs$ 108. $A foreign DNA and plasmid cut by the same restriction end nuclease can be joined to form a recombinant plasmid using$ (1) $Taq polymerase$ (2) $Polymerase III$ (3) $Ligase$ (4) $Eco RI$ 109. $The figure below is the diagrammatic representation of the E. Coli vector pBR322. Which one of the given options correctly identifies its certain component(s)?$ (1) $Ori-original restriction enzyme$ (2) $Rop-reduced osmotic pressure$ (3) $Hind III, EcoRI selectable markers$ (4) $am p^{R}, t e t^{R} antibiotic resistance genes$ 110. $The figure below shows three steps (A, B, C) of Polymerase Chain Reaction (PCR). Select the option giving correct identification together with what it represents? Options:$ (1) $(A): Denaturation at a temperature of about 50ºC.$ (2) $(C): Extension in the presence of heat stable DNA polymerase.$ (3) $(A): Annealing with two sets of primers.$ (4) $(B): Denaturation at a temperature of about 98ºC separating the two DNA strands.$ 111. $A selectable marker is used to:$ (1) $help in eliminating the non-transformants, so that the transformants can be regenerated$ (2) $identify the gene for a desired trait in an alien organism$ (3) $select a suitable vector for transformation in a specific crop$ (4) $mark a gene on a chromosome for isolation using restriction enzyme$ 112. $Spooling is :-$ (1) $Amplification of DNA$ (2) $Cutting of separated DNA bands from the agarose gel$ (3) $Transfer of separated DNA fragments to synthetic membranes$ (4) $Collection of isolated DNA$ 113. $Biolistics (gene-gun) is suitable for$ (1) $Transformation of plant cells$ (2) $Constructing recombinant DNA by joining with vectors$ (3) $DNA finger printing$ (4) $Disarming pathogen vectors$ 114. $What is true about Bt toxin?$ (1) $The concerned Bacillus has antitoxins$ (2) $The inactive protoxin gets converted into active form in the insect gut$ (3) $Bt protein exists as active toxin in the Bacillus$ (4) $The activated toxin enters the ovaries of the pest to sterilise it and thus prevent its multiplication$ 115. $Spooling is:$ (1) $Cutting of separated DNA bands from the agarose gel$ (2) $Transfer of separated DNA fragments to synthetic membranes$ (3) $Collection of isolated DNA$ (4) $Amplification of DNA$ 116. $Transgenic plants are the ones :-$ (1) $Grown in artificial medium after hybridization in the field$ (2) $Produced by a somatic embryo in artificial medium$ (3) $Generated by introducing foreign DNA in to a cell and regenerating a plant from that cell$ (4) $Produced after protoplast fusion in artificial medium$ 117. $Restriction endonuclease -$ (1) $Cuts the DNA molecule randomly$ (2) $Cuts the DNA molecule at specific sites$ (3) $Restricts the synthesis of DNA inside the nucleus$ (4) $Synthesizes DNA$ 118. $Biolistics (gene-gun) is suitable for:$ (1) $Constructing recombinant DNA by joining with vectors$ (2) $DNA finger printing$ (3) $Disarming pathogen vectors$ (4) $Transformation of plants cells$ 119. $In RNAi, the genes are silenced using:$ (1) $ds-RNA$ (2) $ss-DNA$ (3) $ss-RNA$ (4) $ds-DNA$ 120. $The two antibiotic resistance genes on vector pBR322 are :-$ (1) $Ampicillin and Tetracycline$ (2) $Ampicillin and Chloramphenicol$ (3) $Chloramphenicol and Tetracycline$ (4) $Tetracycline and Kanamycin$