Biotechnology: Principles & Processes PYQs Set 3 (PDF)

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Mere Teacher · Previous Year Questions NEET Biology · Biotechnology: Principles & Processes · Set 3 (Q61-90)
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61. $Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells) :-$ (1) $an antifeedant$ (2) $a toxic protein$ (3) $both sense and anti-sense RNA$ (4) $a particular hormone$ 62. $In gene therapy of Adenosine Deaminase (ADA) deficiency, the patient requires periodic infusion of genetically engineered lymphocytes because:$ (1) $Gene isolated from marrow cells producing ADA is introduced into cells at embryonic stages$ (2) $Lymphocytes from patient's blood are grown in culture, outside the body.$ (3) $Genetically engineered lymphocytes are not immortal cells.$ (4) $Retroviral vector is introduced into these lymphocytes.$ 63. $In the process of recombinant DNA technology, the bioreactors are used in$ (1) $Downstream processing$ (2) $Amplification of gene of interest$ (3) $Separation of DNA fragments$ (4) $Production of large quantities of culture$ 64. $During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out$ (1) $Polysaccharides$ (2) $RNA$ (3) $DNA$ (4) $Histones$ 65. $In a mixture, DNA fragments are separated by$ (1) $Restriction digestion$ (2) $Electrophoresis$ (3) $Polymerase chain reaction$ (4) $Bioprocess engineering$ 66. $Golden rice is a promising transgenic crop. When released for cultivation, it will help in$ (1) $Alleviation of vitamin A deficiency$ (2) $Pest resistance$ (3) $Herbicide tolerance$ (4) $Producing a petrol-like fuel from rice$ 67. $Genetically modified organisms (GMO) have been useful in many ways. Which is incorrect about genetic modification?$ (1) $They made crops more tolerant to drought.$ (2) $They enhance the nutritional value of food.$ (3) $They reduce the post harvest losses.$ (4) $They enhance reliance on chemical pesticides.$ 68. $Main objective of production/use of herbicide resistant GM crops is to :-$ (1) $Eliminate weeds from the field without the use of herbicides$ (2) $Encourage eco-friendly herbicides$ (3) $Reduce herbicide accumulation in food articles for health safety$ (4) $Eliminate weeds from the field without the use of manual labour$ 69. $In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?$ (1) $5’ G TATTC 3’; 3’ CATAAG 5’$ (2) $5'GATACT 3^{’}; 3^{’} CTATGA 5^{’}$ (3) $5' GAATTC 3^{’}; 3^{’} CTTAAG 5^{’}$ (4) $5’ CTCAGT 3 ’; 3 ’ GAGTCA 5 ’$ 70. $Which one of the following statement is not true regarding gel electrophoresis technique?$ (1) $Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.$ (2) $The process of extraction of separated DNA strands from gel is called elution.$ (3) $The separated DNA fragments are stained by using ethidium bromide.$ (4) $The presence of chromogenic substrate gives blue coloured DNA bands on the gel$ 71. $Which of the following is not a desirable feature of a cloning vector?$ (1) $Presence of a marker gene$ (2) $Presence of single restriction enzyme site$ (3) $Presence of two or more recognition sites$ (4) $Presence of origin of replicatio$ 72. $Plasmid pBR322 has PstI restriction enzyme site within gene am p^{R} that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E.coli strain$ (1) $it will not be able to confer ampicillin resistance to the host cell.$ (2) $the transformed cells will have the ability to resist ampicillin as well as produce β-galactoside.$ (3) $it will lead to lysis of host cell.$ (4) $it will be able to produce a novel protein with dual ability$ 73. $Polyethylene glycol method is used for :-$ (1) $Energy production from sewage$ (2) $Gene transfer without a vector$ (3) $Biodiesel production$ (4) $Seedless fruit production$ 74. $DNA or RNA segment tagged with a radiactive molecule is called :$ (1) $Clone$ (2) $Plasmid$ (3) $Vector$ (4) $Probe$ 75. $Which one of the following techniques made it possible to genetically engineer living organisms?$ (1) $Hybridization$ (2) $Recombinant DNA techniques$ (3) $X-ray diffraction$ (4) $Heavier isotope labelling$ 76. $The maximum number of existing transgenic animals is of$ (1) $Mice$ (2) $Cow$ (3) $Pig$ (4) $Fish$ 77. $In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is$ (1) $Indian Council of Medical Research (ICMR)$ (2) $Council for Scientific and Industrial Research (CSIR)$ (3) $Research Committee on Genetic Manipulation (RCGM)$ (4) $Genetic Engineering Appraisal Committee (GEAC)$ 78. $The specific palindromic sequence which is recognized by EcoRI is :$ (1) $5' - GGATCC - 3' 3' - CCTAGG - 5'$ (2) $5' - GAATTC - 3' 3' - CTTAAG - 5'$ (3) $5' - GGAACC - 3' 3' - CCTTGG - 5'$ (4) $5' - CTTAAG - 3' 3' - GAATTC - 5'$ 79. $Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?$ (1) $5'_______GAATTC ________3' 3'_______ CTTAAG ________5'$ (2) $5'_______CACGTA ________3' 3'_______ CTCAGT________5'$ (3) $5'_______CGTTCG ________3' 3'_______ ATGGTA _______5'$ (4) $5'_______GATATG ________3' 3'_______ CTACTA ________5'$ 80. $The bacterium Bacillus thuringiensis is widely used in contemporary biology as :-$ (1) $Source of industrial enzyme$ (2) $Indicator of water pollution$ (3) $Insecticide$ (4) $Agent for production of dairy products$ 81. $Which one is a true statement regarding DNA polymerase used in PCR?$ (1) $It is used to ligate introduced DNA in recipient cells.$ (2) $It serves as a selectable marker.$ (3) $It is isolated from a virus.$ (4) $It remains active at high temperature.$ 82. $A genetically engineered micro-organism used successfully in bioremediation of oil spills is a species of:-$ (1) $Pseudomonas$ (2) $Trichoderma$ (3) $Xanthomonas$ (4) $Bacillus$ 83. $In a cell, the separation of DNA strands is brought about by the enzyme DNA helicase, whereas in PCR, the separation of DNA strands is due to :$ (1) $Two sets of Primers$ (2) $Taq DNA polymerase$ (3) $Deoxynucleotides$ (4) $High temperature$ 84. $The two polypeptides of human insulin are linked together by :-$ (1) $Hydrogen bonds$ (2) $Phosphodiester bond$ (3) $Covalent bond$ (4) $Disulphide bridge$ 85. $First discovered restriction endonuclease that always cuts DNA molecule at a particular point by recognising a specific sequence of six base pairs is:$ (1) $EcoR1$ (2) $Adenosine deaminase$ (3) $Thermostable DNA polymerase$ (4) $Hind II$ 86. $Exploitation of bioresources of a nation by multinational companies without authorization from the concerned country is referred to as-$ (1) $Bioweapon$ (2) $Biopiracy$ (3) $Bioethics$ (4) $Biowar$ 87. $The genetically-modified (GM) brinjal in India has been developed for :$ (1) $Enhancing mineral content$ (2) $Drought-resistance$ (3) $Insect-resistance$ (4) $Enhancing shelf lif$ 88. $Genetic engineering has been successfuly used for producing :$ (1) $transgenic Cow-Roise which produces high fat milk for making ghee$ (2) $animals like bulls for farm work as they have super power$ (3) $transgenic mice for testing safety of polio vaccine before use in humans$ (4) $transgenic models for studying new treatments for certain cardiac diseases$ 89. $Some of the characteristics of Bt cotton are:$ (1) $High yield and production of toxic protein crystals which kill dipteran pests$ (2) $High yield and resistance to bollworms$ (3) $Long fibre and resistance to aphids$ (4) $Medium yield, long fibre and resistance to beetle pests$ 90. $For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of$ (1) $Silver or Platinum$ (2) $Platinum or Zinc$ (3) $Silicon or Platinum$ (4) $Gold or Tungsten$