NEET Previous Year Question

An analysis of chromosomal DNA using the Southern hybridization technique does not use

Topic: NEET PYQ

Answer: D. Full step-by-step solution below.

  1. AElectrophoresis
  2. BBlotting
  3. CAutoradiography
  4. DPCR

Correct answer: D

Solution

(d) Southern hybridization involves electrophoresis, blotting, hybridization with a labeled probe, and autoradiography for detection. PCR is a separate technique used to amplify DNA and is not a step in Southern hybridization.

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