NEET Previous Year Question
Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection using autoradiography because:
Topic: NEET PYQ
Answer: B. Full step-by-step solution below.
- AMutated gene completely and clearly appears on a photographic film.
- BMutated gene does not appear on a photographic film as the probe has no complimentarity with it.
- CMutated gene does not appear on photographic film as the probe has complementarity with it.
- DMutated gene partially appears on a photographic film.
Correct answer: B
Solution
(b) A single stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography. The clone having the mutated gene will hence not appear on the photographic film, because the probe will not have complementarity with the mutated gene.More Biotechnology & its Applications PYQs
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