31.In the hexaploid wheat, the haploid (n) and basic (x) numbers of chromosomes are :-- (1)n = 7 and x = 21
- (2)n = 21 and x = 21
- (3)n = 21 and x = 14
- (4)n = 21 and x = 7
32.Which scientist experimentally proved that DNA is the sole genetic material in bacteriophage?- (1)Beadle and Tautum
- (2)Messelson and Stahl
- (3)Hershey and Chase
- (4)Jacob and Monod
33.Which of the following features of genetic code does allow bacteria to produce human insulin by recombinant DNA technology?- (1)Genetic code is not ambiguous
- (2)Genetic code is redundant
- (3)Genetic code is nearly universal
- (4)Genetic code is specific
34.Molecular basis of organ differentiation depends on the modulation in transcription by:- (1)RNA polymerase
- (2)Ribosome
- (3)Transcription factor
- (4)Anticodon
35.Which one of the following is the sequence on corresponding coding strand, if the sequence on mRNA formed is as follow 5'AUCGAUCGAUCGAUCGAUCGAUCG AUCG 3?- (1)3' ATCGATCGATCGTCGATCG ATCGATCG 5,
- (2)5 ' UAGCUAGCUAGCUAGCUA GCUAGC UAGC 3'
- (3)3' UAGCUAGCUAGCUAGCUA GCUAGCUAGC 5'
- (4)5 ' ATCGATCGATCGATCGATCG ATCGATCG 3,
36.E. Coli has only 4.6×106 base pairs and completes the process of replication within 18 minutes; then the average rate of polymerisation is approximately-- (1)3000 base pairs/second
- (2)4000 base pairs/second
- (3)1000 base pairs/second
- (4)2000 base pairs/second
37.A complex of ribosomes attached to a single strand of RNA is known as :-- (1)Polysome
- (2)Polymer
- (3)Polypeptide
- (4)Okazaki fragment
38.Choose the correct pair from the following:- (1)Polymerases
- Break the DNA into fragments - (2)Nucleases
- Separate the two strands of DNA - (3)Exonucleases
-
Make cuts at specific positions within DNA - (4)Ligases
- Join the two DNA molecules
39.If the length of a DNA molecule is 1.1 metres, what will be the approximate number of base pairs?- (1)6.6 × l09 bp
- (2)3.3 × 106 bp
- (3)6.6 × 106 bp
- (4)3.3 × l09 bp
40.DNA fingerprinting involves identifying differences in some specific regions in DNA sequence, called as :- (1)Satellite DNA
- (2)Repetitive DNA
- (3)Single nucleotides
- (4)Polymorphic DNA
41.What is the role of RNA polymerase III in the process of transcription in Eukaryotes?- (1)Transcription of only snRNAS
- (2)Transcription of rRNAs (28S,18S and 5.8S)
- (3)Transcription of tRNA, 5 srRNA and snRNA
- (4)Transcription of precursor of mRNA
42.Ten E. coli with 15N - dsDNA are incubated in medium containing 14N nucleotide. After 60 minutes, how many E. coli cells will have DNA totally free from 15N ?- (1)80 cells
- (2)20 cells
- (3)40 cells
- (4)60 cells
43.What is the criterion for DNA fragments movement on agarose gel during gel electrophoresis?- (1)The smaller the fragment size, the farther it moves
- (2)Positively charged fragments move to farther end
- (3)Negatively charged fragments do not move
- (4)The larger the fragment size, the farther it moves
44.Ten E.coli cells with 15N - dsDNA are incubated in medium containing 14N nucleotide. After 60 minutes, how many E.coli cells will have DNA totally free from 15N?- (1)40 cells
- (2)60 cells
- (3)80 cells
- (4)20 cells
45.Which is the basis of genetic mapping of human genome as well as DNA fingerprinting?- (1)Single nucleotide polymorphism
- (2)Polymorphism in hnRNA sequence
- (3)Polymorphism in RNA sequence
- (4)Polymorphism in DNA sequence
46.If Adenine makes 30% of the DNA molecule, what will be the percentage of Thymine, Guanine and Cytosine in it?- (1)T:20;G:20;C:30
- (2)T:30;G:20;C:20
- (3)T:20;G:25;C:25
- (4)T:20;G:30;C:20
47.Antiparallel strands of a DNA molecule means that- (1)one strand turns anti-clockwise
- (2)the phosphate groups of two DNA strands, at their ends, share the same position
- (3)the phosphate groups at the start of two DNA strands are in opposite position (pole)
- (4)one strand turns clockwise
48.What is it that forms the basis of DNA Fingerprinting?- (1)The relative difference in the DNA occurrence in blood, skin and saliva.
- (2)The relative amount of DNA in the ridges and grooves of the fingerprints.
- (3)Satellite DNA occurring as highly repeated short DNA segments.
- (4)The relative proportions of purines and pyrimidines in DNA.
49.Commonly used vectors for human genome sequencing are :-- (1)T-DNA
- (2)BAC and YAC
- (3)Expression Vectors
- (4)T/A Cloning Vectors
50.Which of the following RNAs is not required for the synthesis of protein?- (1)tRNA
- (2)rRNA
- (3)siRNA
- (4)mRNA
51.PCR and Restriction Fragment Length Polymorphism are the methods for- (1)Study of enzymes
- (2)Genetic transformation
- (3)DNA sequencing
- (4)Genetic fingerprinting
52.DNA replication is semi-conservative in nature was experimentally proved in eukaryotes by :- (1)Macleod and McCarty
- (2)Meselson and Stahl
- (3)Talyor and his colleagues
- (4)Hershey and Chase
53.All of the following are part of an operon except :-- (1)an operator
- (2)structural genes
- (3)an enhancer
- (4)a promoter
54.Which of the following is not required for any of the techniques of DNA fingerprinting available at present?- (1)Polymerase chain reaction
- (2)Zinc finger analysis
- (3)Restriction enzymes
- (4)DNA–DNA hybridization
55.Which one of the following does not follow the central dogma of molecular biology?- (1)Chlamydomonas
- (2)HIV
- (3)Pea
- (4)Mucor
56.The final proof for DNA as the genetic material came from the experiments of :- (1)Hershey and Chase
- (2)Avery, Mcleod and McCarty
- (3)Hargobind Khorana
- (4)Griffith
57.DNA-dependent RNA polymerase catalyzes transcription on one strand of the DNA which is called the :-- (1)Alpha strand
- (2)Antistrand
- (3)Template strand
- (4)Coding strand
58.Which one of the following is wrongly matched?- (1)Transcription – Writing information from DNA to tRNA.
- (2)Translation – Using information in mRNA to make protein.
- (3)Repressor protein – Binds to operator to stop enzyme synthesis.
- (4)Operon – Structural genes, operator and promoter.
59.Which of the following statements is correct?- (1)Adenine pairs with thymine through one H -bond
- (2)Adenine pairs with thymine through three H-bonds.
- (3)Adenine does not pair with thymine.
- (4)Adenine pairs with thymine through two H -bonds.
60.Select the correct option: ((A): Direction RNA synthesis, (B): Direction of reading of the template DNA strand)- (1)5′ ----- 3′ 3′ ------ 5′
- (2)3′ ----- 5′ 5′ ------ 3′
- (3)5′ ----- 3′ 5′ ------ 3′
- (4)3′ ----- 5′ 3′ ------ 5′
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