NEET Previous Year Question

The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of

Topic: NEET PYQ

Answer: C. Full step-by-step solution below.

  1. ANon-recombinant bacteria containing β-galactosidase.
  2. BInsertional inactivation of β-galactosidase in non-recombinant bacteria.
  3. CInsertional inactivation of β-galactosidase in recombinant bacteria.
  4. DInactivation of glycosidase enzyme in recombinant bacteria.

Correct answer: C

Solution

(c) In recombinant bacteria, the inserted DNA disrupts the lacZ (β-galactosidase) gene, so β-galactosidase is not produced and the colonies appear white. Non-recombinant bacteria produce β-galactosidase and form blue colonies.

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